SmurfTrsc for FlyBase

Smurf vs non-Smurf and age gene expression from Zane et al. 2023 (Aging Cell) on FlyBase gene, protein, transcript and allele pages.

SmurfTrsc for FlyBase shows the whole-body transcriptome of Smurf and non-Smurf Drosophila melanogaster females (Zane et al., Aging Cell, 2023, doi:10.1111/acel.13946) directly on FlyBase report pages, so you can see how a gene behaves across the two phases of ageing without leaving the page you are reading. On gene (FBgn), protein (FBpp), transcript (FBtr) and allele (FBal) pages, a panel at the top of the report shows: • a one-line verdict: up, down or no change in Smurfs, with log2 fold change and adjusted p, and the paper's category (Smurf gene, old non-Smurf gene, or both); • the 32 RNA-seq samples as a strip plot by age (20, 30, 40 days) and Smurf status, with group means; hover a sample for its details, or open the table of all values; • the three DESeq2 contrasts of the paper: Smurfs vs non-Smurfs, and 40 vs 20 days within each status, so Smurf status and age can be told apart; • where the gene has them, the paper's other results: longevity candidates, the linear model on expression, progressive change in non-Smurfs, proteomics, GenAge lifespan effects and the Human Ageing Atlas ortholog. The dataset ships inside the extension: it makes no network requests, needs no account and collects nothing. Protein, transcript and allele pages show their parent gene. Works alongside ProteoCast for FlyBase. More: https://michaelrera.github.io/tools/smurftrsc-flybase/ Source code: https://github.com/MichaelRera/smurftrsc-flybase Source data: https://github.com/MichaelRera/SmurfsTrsc Not affiliated with FlyBase.